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For this, we introduced a second copy of gspD under the control of the copper-inducible promoter, P cuoA at the attB site 75 into our clones that had successfully integrated the deletion plasmid

In clinical trials, neither weight gain nor weight loss was consistently reported as a side effect

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Most trials showing benefit used multi-strain formulations alongside caloric restriction, making it difficult to isolate probiotic effects

Age, baseline metabolic health, and concurrent medications can also affect tolerance

One in three participants lost 20% or more of their starting weight