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Because chronic inflammation is strongly associated with metabolic dysfunction, this mechanism has generated interest among researchers studying metabolic disease

Peptides were prepared at 100 g/mL and serially diluted 2-fold in Mueller-Hinton Broth (MHB)

Never freeze the reconstituted solution, protect it from light, and discard if it turns cloudy or shows particles

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That's not a failure of scientific rigor

Blood samples were collected by venipuncture in Vacutainer CPT Mononuclear Cell Preparation Tubes (BD, Franklin Lakes, NJ) with sodium heparin, immediately placed in ice water, and then centrifuged at 2,000 g at 4C for 10 min