The peptide does not exhibit significant tachyphylaxis (receptor desensitization) in short-to-medium-term studies spanning 1428 days
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Hormone measurements Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to determine the serum concentration of leptin (Crystal Chem), T4 (Calbiotech), testosterone (Calbiotech), corticosterone (Arbor Assays), GH (Millipore), IGF-1 (R&D Systems) and prolactin (Sigma)
These findings were reversible and resolved during the recovery period
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