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After three basal measurements, cells were permeabilized by injection of saponin (1 g/mL, Sigma S4521), together with 1 mM ADP (Sigma 01905) and the following respiratory complex substrates: CI, pyruvate/malate (5 mM/2.5 mM)
Staying hydrated and avoiding rapid position changes helps manage this effect
This phenomenon has been attributed to the reliance of non-tumor epithelial and mesenchymal cells on a rigid surface for survival [26]
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631 However, similar results were not found in a 4-week phase 2a study involving patients with PDPN
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