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If you use only 1 mL you get 10 mg/mL and every number halves, but your 0.25 mg starting dose becomes 2.5 units, which is too small to draw accurately on a U-100 barrel

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Handling and Storage Recommendations To preserve sample integrity and support safe laboratory practices, the following guidelines should be followed: Store at 20 C or below (non-frost-free preferred)

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A healthcare professional will then collect a blood sample from a vein in your arm for analysis