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Ratiometric time-course measurements were carried out with initially oxidized or reduced roGFP2 on a BMG POLARstar Omega fluorescence plate reader with a filter-based excitation at 39010 and 48010 nm

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To confirm these observations, purified PDI was treated with different concentrations of 4-HNE in vivo (20200 M) to show a 1456% inhibition, respectively

doi:10.2174/0115734064335901241101025220