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e , Bar graph showing fractions of proteins in ER-IP, whole cell and control-IP samples from HEK293T expressing ER-tag or control-tag

In that case, self-injection method is a feasible way which can be easily done by following the underlying steps: Woman performing self-injection- Picture Courtesy: gonoble.com Step 1: Gather Necessary Tools Wash and dry your hands properly

The native gel was freshly poured and contained 4.5% (w/v) acrylamide and bis-acrylamide (29:1), 2% (v/v) glycerol, TB buffer, TEMED (75 l in 100 ml, gel solution) and 0.04% (w/v) APS

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