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Tissues were permeabilized with PBS supplemented with 0.1% Tween-20 for 10 min, followed by a peroxidase block with 3% H 2 O 2 (Sigma-Aldrich, 216763) for 20 min
10.1016/j.cbpc.2008.10.108 Compar
One-meal-per-day eaters are actually ideal candidates for cagrilintide timing optimization because there's no ambiguity about which meal to target
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