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For vibratome-cut sections, 4% paraformaldehyde-fixed brains were cut into 50 m-thick sections by a vibratome

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Wed rather have that conversation directly with you than publish a generic protocol that may not reflect whats actually appropriate for your situation

Islam T, Manna M, Kaul T, Pandey S, Reddy CS, Reddy MK (2014) Genome-wide dissection of Arabidopsis and rice for the identification and expression analysis of glutathione peroxidases reveals their stress-specific and overlapping response patterns

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Glutathione peroxidase catalyzes reactions in which the enzyme reduces hydrogen peroxide to water, as well as organic hydroperoxides (ROOH) to hydroxy derivatives, resulting in the conversion to the oxidized disulfide form GS-SG: H2O2 + 2GSH = GS-SG + H2O ROOH + 2GSH = GS-SG + ROH +H2O Glutathione peroxidase neutralizes peroxynitrite: ONOO- + 2GSH N2- + GSSG + 22 Glutathione reductase is a flavoprotein with a flavin adenine dinucleotide prosthetic group and consists of two identical subunits