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Electrostatic potentials for ScGrx6 and ScGrx7 were computed and mapped to the protein surface using the PoissonBoltzmann method of Swiss-PDB Viewer (dielectric constant solvent: 80, atomic partial charges, dielectric constant protein: 4, solvent ionic strength: 0.1 M)

To determine differences among patient groups, categorical variables were compared by chi-squared analysis, continuous variables with t -tests, and, if skewed, with non-parametric tests (MannWhitney U -test)

Indeed, SH-SY5Y cells showed high GSH levels but exhibited very low GPx activity, whereas AGS cells had low GSH content but several isoforms of the GPx enzyme

To simulate extreme acidic conditions, PBS (pH=1) was used as the positive control group (PC)

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Depending on client preference and the specific treatment goals, Vitamin C may be combined with the glutathione therapy