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Importantly, microglia in this activated state also secrete pro-inflammatory cytokines such as TNF-, IL-1, and IL-6, which further amplify ROS production and reinforce the inflammatory state

Emdin, M., Pompella, A

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Cell Rep 11(4):577591 Kamba T, McDonald DM (2007) Mechanisms of adverse effects of anti-VEGF therapy for cancer

Repair the barrier first, then introduce copper peptides

At concentration ranges typical to published preclinical protocols, the copper mass delivered by GHK-Cu is orders of magnitude below established acute toxicity thresholds