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Fluorescent staining for casepase3/7 was done using CellEvent TM Caspase-3/7 Green Detection Reagent Kit (Thermo Fisher Scientific)

Regarding prescribing: under the Human Medicines Regulations 2012 (regulation 167), doctors and some independent prescribers may prescribe unlicensed medicines (known as 'specials') when clinically necessary and when no licensed alternative is suitable, taking personal responsibility for that decision (GMC: Good practice in prescribing and managing medicines and devices)

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After 10 min of administration, the total radioactivity concentration increased significantly in all tissues, with the mean renal tissue concentration reaching 223 ng (Eq