RNA extraction and qRTPCR analysis Cells were grown to 80% confluency in 75 cm 2 flasks, harvested by trypsinisation, counted using a haemocytometer, and 1 10 7 cells used for RNA extraction using a Qiagen RNeasy mini kit (Qiagen), following the manufacturers protocol for mammalian cells, with additional on column DNAse digestion (RNAse free DNAse kit, Qiagen)
All treatments continued for eight weeks 63
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Abstract Effects of combination therapy of dipeptidyl peptidase-4 (DPP-4) inhibitor and sodium-glucose co-transporter 2 (SGLT2) inhibitor on -cells are still unclear, although combination agent of these two drugs has become common in clinical practice
However, "general population" does not always account for the unique physiological state of pregnancy
Desiraju GR, Steiner T: The Weak Hydrogen Bond (IUCr Monograph on Crystallography