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Nitrogen is the limiting factor for biomass formation in the composition of the M9 medium, which is why NH 4 Cl was supplemented in this experiment to double the concentration to 2 g L 1 to reach a higher biomass in this cultivation

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Sirrs S, Munk P, Mallinson PI, Ouellette H, Horvath G, Cooper S, Da Roza G, Rosenbaum D, ORiley M, Nussbaumer G, Hoang LN, Lee CH (2014) Cystinosis with sclerotic bone lesions