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Samples were then amplified and sequenced on a NextSeq 500 system (Illumina), where an average of 30 million reads were generated from each sample

This was one of the goals of its design, leading to a longer half-life in laboratory settings

Thus, we found that a distinct subset of proteins are targets for persulfide accumulation in wild-type, GR-null, or TR/GR-null liver samples, suggesting that the persulfide-reducing capacities of the Trx and GSH systems might be protein persulfidespecific and potentially involved in different signaling pathways

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