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RNA interference To silence GLP-1R and PKA gene expression, cells were transfected with siRNAs using lipofectamine RNAi MAX reagent (Invitrogen)

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Results vary based on: Starting weight Metabolic factors Consistency with treatment Most are mild and temporary: Nausea (usually subsides in weeks) Constipation Headaches Severe side effects are rare but include pancreatitis or gallbladder issues

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