Aliquots of the cDNA were used to measure the expression levels of the genes with the primers, and Power SYBR Green master mix (Applied Biosystems, ThermoFisher Scientific) on a 7900 HT sequence detection system (Applied Biosystems, ThermoFisher Scientific)
Patients were assessed at baseline and after 3 months of starting KD, the assessments of children included: 24-hour dietary recall, Chalfont Seizures Severity Scale, gastrointestinal symptoms score and Bristol stool chart, frequency of defecation per week, anthropometric measurements assessment, fasting serum lipid profile and measurement of the antral length by ultrasound
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