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One possible caveat with procedure B is that in the absence of IAM/IAM-TPP as a capping agent, extended exposure to TCEP could sequester internal sulfane sulfur atoms as TCEP = S 44 through nucleophilic attack on the internal sulfur atom (Supplementary Fig
However, further studies are needed to confirm its efficacy and long-term benefits
Simple as that
Technol Cancer Res Treat
Intracellular delivery of glutathione S-transferase-fused proteins into mammalian cells by polyethylenimine-glutathione conjugates
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