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Approximately 3000 PGCs per sample were transferred into 1.5-mL tubes and resuspended in 60 L of wash buffer (20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM spermidine, and protease inhibitors)
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Somatic mutations are frequent and increase with age in human kidney epithelial cells
Garbage in, garbage out
Consistently, similar results were obtained in Trim37 cko embryos
This recessive disorder has stayed within the genotypes of humans most probably because the deficiency has been found to protect against a very lethal form of malaria
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