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Replacement with Trp 7 lowers the affinity (IC 50 : 0.26 nM3.3 nM) and strongly lowers the activity (EC 50 : 0.8 nM127 nM)

Some self-tests require this type of repeat testing in the manufacturers instructions

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(3) Ensure the levels of micro-nutrients such as vitamin C and D to support collagen synthesis and bone metabolism