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The resulting plasmid was linearised by AflII digestion (NEB) and transformed into PichiaPink Strain one (Invitrogen, USA) by electroporation

Sonication involves exposing samples to ultrasonic waves which agitate the molecules and increase the solubility of peptide compounds in solution

however, in conditions where there is a high demand for nucleotide production (such as in the case for highly proliferative cells), the nonoxidative part of the pathway can function independently of the oxidative phase to produce ribose 5-phosphate from the glycolytic intermediates fructose 6-phosphate and glyceraldehyde 3-phosphate (figure 7.2)

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The altered relationship with food and hunger cues can be destabilizing