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This includes understanding the molecular stability, appropriate storage conditions, and handling procedures to prevent degradation and contamination, thereby ensuring reliable and reproducible results in preclinical research

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Proteomic analysis Cells were seeded in 10-cm dishes and cultured for 7 days in cystine-free medium supplemented with heavy isotope-labelled 13 C 6 , 15 N 2 -cystine (Cambridge Isotope Laboratories, CNLM-4244-H)

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Peptides were injected onto a 75 m 2 cm trap column (Thermo Fisher) before re-focusing on an Aurora Elite 75 m 15 cm C18 column (IonOpticks) using a Vanquish Neo UHPLC (Thermo Fisher) connected to an Orbitrap Astral mass spectrometer (Thermo Fisher)