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HEK293T cells were grown to 70% confluency in 96-well plates (Corning) at 37C in DMEM supplemented with 10% FBS, and treated with Entranster TM -H (Engreen) transfection reagent (0.1 L) and purified plasmids [0.25 g pGL4.74 (hRluc/TK) and 0.25 g pGL4.32(luc2P/NF-B-RE/Hygro plasmid)] in 50 L DMEM/10% FBS per well
PMID 12095224
D.ForcinaG
4 Thus, defects in SLC25A39 induce severe disturbances in the mitochondrial GSH-import machinery contributing to neuronal degeneration
Environ Sci Pollut Res 27:1022810235
Reported effects are generally mild injection-site reactions, fatigue, nausea, lightheadedness
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