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Determination of cellular thiols and glutathione-related enzyme activities: versatility of high-performance liquid chromatographyspectrofluorimetric detection

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First, its molecules are small (228 Da) and very lipophilic (oil loving) and these properties result in very good absorption

The gastrocnemius muscles were then excised, washed in cold phosphate-buffered saline, snap-frozen in liquid nitrogen-cooled isopentane, and stored at 80 C until analysis [2]