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If you freeze something, the structure of the peptides changes because ice crystals form

Figure 2 The viability results of frozen-thawed spermatozoa, shown in Figure 2B, were partially consistent with the motility results of frozen-thawed spermatozoa as viability in the GSH + SeNPs group (69.44 3.24%) was significantly higher than that in the NC (56.33 7.43%), GSH (61.89 4.35%) and SeNPs (63.49 3.23%) groups ( P 0.05)

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Rossi and Freeman say

These pathways are connected to blood vessel formation, tissue oxygenation, and cellular repair