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Qiu et al., 2023)

Copper can catalyze oxidation reactions

The first injection in port A contained the substrate for each individual respiratory chain complex (I: 5 mM pyruvate + 2.5 mM malate, II: 10 mM succinate + 1 M rotenone, III: 0.5 mM duroquinol, IV: 0.5 mM TMPD + 2 mM ascorbic acid) along with 1 mM ADP and 10 nM recombinant perfringolysin O (Agilent Technologies)

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The G of G/D-CuP decreased from 2500 Pa (0.1% strain) to 390 Pa (1200% strain), but upon strain relief, the G recovered to 2500 Pa (Fig