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The lysate was applied to a HisTrap column (Cytiva), washed with 10 column volumes (CV) of wash buffer (50 mM Tris pH 7.5, 500 mM NaCl, 50 mM Imidazole) and eluted with 5 CV of elution buffer (50 mM Tris pH 7.5, 500 mM NaCl, 500 mM Imidazole)

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Never freeze the vial: freezing damages the peptide structure and makes it ineffective

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Figure 1: Changes in gross morphological scores amongst the four experimental treatment groups