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For lentiviral induction (Day-0), 1 g/mL doxycycline (Sigma-Aldrich, Australia) was added to neural induction media [Neurobasal medium (Thermo, Australia), 1 N-2 supplement (Thermo, Australia), 1 B-27 with vitamin A supplement (Thermo, Australia), 1 Insulin-transferrin-Selenium-A (Thermo, Australia), 1 GlutaMAX TM (Life Technologies, USA)] supplemented with 10 M SB431524 (Stemcell Technologies, Canada) and 0.1 M LDN193189 (Stemcell Technologies, Canada)

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