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See GL, Arce F Jr, Dahlizar S, Okada A, Fadli M, Hijikuro I, et al

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HEK293T cells were transfected with Flag-EAAT3, V5-SLC7A11, and pcDNA3.1-Vector through PEI (plasmid : PEI 1:4) transfection reagents, which replaced the medium after 46 h

Together these modifications reduce IGFBP binding affinity by approximately 1,000-fold compared to native IGF-1 while preserving IGF-1 receptor (IGF1R) binding

What Does the Research Actually Show

Methods Reagents All chemicals and reagents were purchased from Sigma-Aldrich (St