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Then, the free Cys residues of GSAAT At molecules in the leaf extract were labeled with the sulfhydryl-binding reagent 1mM 4-acetoamido-4-maleimidylstilbene-2,2-disulfonic acid (AMS), and subsequently separated by non-reducing denaturing sodium dodecyl sulfatePA gel electrophoresis (SDS-PAGE)

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Each receptor contributes distinct metabolic effects

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Dosing here comes from preclinical animal research (Neelakantan et al., 2018 onward) and community-reported protocols