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These are generally mild and self-limiting

peptide sequence or chemical identity

The supernatant was collected and stored at 80 C

Extremophilic bacterial GGTs exhibit only hydrolytic activity suggesting them to be the ancient progenitors that evolved earliest with only hydrolytic activity, and later, transpeptidase activity was imparted to other mesophilic bacterial and eukaryotic GGTs ( Biochemical properties of prokaryotic GGTs are not very diverse, and they optimally function at alkaline pH ranging from 8.0 to 11.0 with different pH optima for hydrolysis and transpeptidation reactions (Morelli et al., 2014)

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