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Finally, leaked electrons inevitably binds to the oxygen molecules and produces oxygen free radical and non-radical species in mitochondria as well as cytosol [25]

This leads to a faster breakdown of glutathione (GSH), a key antioxidant

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NADPH in aqueous solution excited at the nicotinamide absorbance of ~335 nm (near UV) has a fluorescence emission which peaks at 445-460 nm (violet to blue)

Among them, the Ser-65 residue was generally conserved across all GST classes