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The peptide was acquired as a 99% pure substance that was further dissolved in saline prior to the random assignment of male mice to an experimental treatment

The lack of statistically significant reduction in infarct volume, despite a numerical decrease, suggests that DSIPs primary benefit in this model might be more related to functional neurorecovery and neural network plasticity rather than a direct, massive reduction of necrotic tissue [1]

Phosphate-buffered saline (PBS) or other buffered solutions may be appropriate depending on the experimental system

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Do not inject the leftover medicine

Do chronic diseases play a role, such as anemia in chronic kidney disease