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Whole-mount embryo immunostaining E9.5 embryos ( Trim37 ko1 and Trim37 cko ) were harvested, fixed in 4% paraformaldehyde at 4 C for 2 h, and washed twice with PBS

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From fermentation to purification, every step meets GMP despite the molecules complexity

doi: 10.1016/j.ccell.2022.02.003 111 ZhaoMvan StratenDBroekmanMLDPratVSchiffelersRM

Experimental: 2.38 0.19

FGF21 analogs have not so far employed up-titration, but given the reportedly mild-to-moderate as well as the transient nature of the nausea and diarrhea, such an approach could reasonably be expected to mitigate the GI side effects