Following the injection, imaging was performed using an IVIS Spectrum system (PerkinElmer) at various time points: 1 h, 4 h, 7 h, 12 h, 24 h, 36 h, 48 h, and 60 h post-injection, with an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm
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For global health applications, room-temperature stability is particularly important: it enables distribution in tropical climates without the cold-chain infrastructure that limits access to injectable biologics in much of the developing world
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(F) TGR5, has been reported equally distributed along the whole gastrointestinal tract of dogs (272, 273)