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10.1038/415096a 12 FengY.FanJ.LinX.YangJ.CuiQ.TangX.et al (2017)

Yousefi, S., Mihalache, C., Kozlowski, E., Schmid, I

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Highly purified water (typically water for injection, which has undergone multiple distillation or reverse osmosis) is mixed with a precisely measured amount of benzyl alcohol so that the resulting concentration is exactly 0.9% (weight/volume)

Total cellular extracts (200 g of protein) were incubated with an anti-IKK- antibody (1 g/mL) in IP buffer overnight at 4 C on a rocker

Repeat every 23 months in severely deficient areas, or every 56 months where deficiency is mild