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Sholl analysis, performed with a plugin in ImageJ software (NIH, version 1.53c.), automatically drew concentric circles from the center of the DAPI signal outward to the ends of the astrocyte processes, analyzing the number of intercepts and the ramification index

MC3R: Found in various tissues, including the brain and placenta, MC3R has been implicated in regulating appetite and maintaining energy homeostasis

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Environmental controls prevent degradation that can affect experimental outcomes