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All starting cultures were taken from cultures grown overnight inside the anaerobic chamber, and MICs were based on their aerobic values

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Then the tubes from positions 2, 3, 6, 7, and 8 were filled with GST P1-1, phosphate buffer pH 6.5, inhibitor, GSH, and CDNB, respectively

Cyanocobalamin is the most widely available synthetic form, is shelf-stable, and is FDA-approved for treating B12 deficiency