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Based on a genome comparison of the most immunogenic T cell antigens for HCMV, HHV-6, -7 and their EEHV orthologues 13,14,15,16,17,27 , a literature review and a study that aimed to identify immunogenic EEHV T cell targets 28 , the major capsid protein (MCP) and the EEHV-specific, putative regulatory protein EE2 were selected as EEHV vaccine candidate antigens

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Finally, the membrane was incubated in SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific Pierce Protein Biology) and imaged with ChemiDocTMTouch Imaging system (BioRad)

doi:10.3390/ijms161226183 Wu N, Yang M, Gaur U, Xu H, Yao Y, Li D