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In vitro studies on bone cells supported the expression of the conventional GLP-1R in osteoblasts and confirmed that liraglutide, at nanomolar concentrations, promoted osteoblast differentiation and reduced osteoblast apoptosis via activation of the PI3K/Akt/GSK3K, MAPK, and cAMP/PKA/-catenin intracellular pathways [46,47,48]

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For frequent blood sampling one day ante injectionem (ai) until the sampling at 12 h post injectionem (pi) , cows previously received an indwelling venous catheter (2.4 mm x 200 mm Teflon catheter, Walter Veterinr-Instrumente e.K., Baruth/Mark, Germany) in the contralateral V

doi: 10.1016/j.puhe.2016.12.033 17 CarterARSandersonEHammertonGRichmondRCDavey SmithGHeronJet al