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During transit down the descending limb more water is reabsorbed and up the ascending limb ions (e.g

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Embryo and cell immunostaining For section immunostaining, E9.5 embryos were collected, washed with cold PBS, fixed in 4% paraformaldehyde overnight at 4 C, and dehydrated through a graded sucrose series (10%, 20%, and 30%

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