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Cell lysate was centrifuged and the soluble roGFP proteins purified via a Ni 2+ loaded HiTrap Chelating HP Column (GE Healthcare, www.gelifesciences.com)

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Inject on the same day each week

GLP-1 RA Addition to Background Sulfonylurea Therapy Similar to the discussion above about background insulin, it is prudent in many cases to hold or reduce the dose of background sulfonylurea therapy (an initial 50% dose reduction is reasonable in many patients) when starting a GLP-1 RA