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All experimental protocols involving animal use were meticulously designed and conducted following ethical guidelines, and approval was obtained from the Animal Experiment Ethics Committee of Guizhou University of Traditional Chinese Medicine (SYXK (Qian) 2021-0005)
Eklund et al., 2007
Use proper needle technique by pinching the skin, inserting the needle at a 90 angle (or 45 if your skin is very thin), and choosing smaller-gauge needles for less discomfort
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Higher concentrations reduce injection volume but increase measurement errortargeting 5mg/mL allows precise dosing with standard insulin syringes calibrated in 0.01mL increments
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