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Abbreviations SCI: Spinal cord injury NSCs: Neural stem cells AMPK: Adenosine 5-monophosphate-activated protein kinase Glutathione peroxidase 4: GPX4 SLC7A11: Solute carrier family 7 member 11 ACSL4: Acyl-coenzyme A synthetase long-chain family member 4 GSH: Glutathione ROS: Reactive oxygen species MMP: Mitochondrial membrane potential HE: Hematoxylin-eosin BBB: Basso, Beattie, and Bresnahan LSS: Louisville swim scale MEP: Motor evoked potentials References Huang H, Young W, Skaper S, Chen L, Moviglia G, Saberi H, Al-Zoubi Z, Sharma H, Muresanu D, Sharma A, et al

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BPC-157 Reconstitution For a 5mg vial: Add 2.5mL of bacteriostatic water (BAC water) Concentration = 2mg/mL (or 2000mcg/mL) For a 250mcg dose: draw 12.5 units (0.125mL) on a U-100 insulin syringe For a 500mcg dose: draw 25 units (0.25mL) For a 10mg vial: Add 5mL BAC water Concentration = 2mg/mL (same math as above) TB-500 Reconstitution For a 5mg vial: Add 2.5mL BAC water Concentration = 2mg/mL For a 2.5mg dose: draw 125 units (1.25mL) this is a larger volume injection Consider splitting into two 62.5 unit injections if volume is uncomfortable For a 10mg vial: Add 2mL BAC water Concentration = 5mg/mL For a 2.5mg dose: draw 50 units (0.5mL) much more manageable Pro tip: TB-500 often comes in 5mg or 10mg vials

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Environ Toxicol Chem 29:730741 Armknecht SL, Kaattari SL, Van Veld PA (1998) An elevated glutathione S -transferase in creosote-resistant mummichog ( Fundulus heteroclitus )

The combined cost of CagriSema may exceed the cost of retatrutide monotherapy, even though retatrutide requires higher individual doses