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Twin-Strep-tagged ligands were captured on the biosensors using the following protocol: Baseline 1: 120 s in running buffer Ligand loading: 120 s (target shift 0.51.0 nm) Baseline 2: 200 s in running buffer The running buffer consisted of 50 mM HEPES, 100 mM NaCl, and 0.5% Triton X-100 at pH 7.4

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doi: 10.3389/fendo.2020.00365 Polat, I.H., Tarrado-Castellarnau, M., Bharat, R., Perarnau, J., Benito, A., Cortes, R., Sabatier, P., and Cascante, M

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