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A reaction mixture (GSSG) was made by combining 2 mM EDTA, 0.15 mM NADPH, 50 mM buffer solution of potassium phosphate (pH 7.8), 0.2 ml enzyme extract, and 0.5 mM oxidized glutathione
10.1186/s13229-021-00472-4 49 EmanueleE.OrsiP.BosoM.BrogliaD.BrondinoN.BaraleF.et al (2010)
Agar gels were diluted with 1 mL PBS, melted at 85 C for 3 min, and centrifuged at 40 C (5 min at 1800 g ) to pellet the cells
Let it dry completely before injecting