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A valid COA must include two specific types of analytical chromatography: High-Performance Liquid Chromatography (HPLC): This determines the purity of the compound
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Proton magnetic resonance spectroscopy ( 1 H MRS), with its extremely high sensitivity, is used for detecting and quantifying several endogenous tissue metabolites in a single acquisition, but it is not capable of tracking the activity of a metabolic pathway due to limitations related to water, lipid suppression, and magnetic field homogeneity [22]
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