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In individuals with Type 2 Diabetes (T2D), there is an observed increase in Gram-negative Enterobacteriaceae and a concurrent decrease in acetic acid-producing bacteria, such as Bifidobacteria

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VPA treatment was discontinued and L-carnitine supplementation (100 mg/kg per day) was initiated

Subsequently, an oral formulation for once-daily administration (Rybelsus , Novo Nordisk) was developed in 2019 to treat type 2 diabetes

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Postnatal cortical neuron isolation and culture Postnatal cortical neurons were isolated following a protocol adapted from Brewer and Torricelli (2007) and the Miltenyi Biotec MACS protocol for the isolation and cultivation of neurons from adult mouse brain