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whether they are present on alpha or delta cells is controversial (49, 88)
Cell-based studies should also account for cell type, passage number, serum conditions, culture density, assay timing, and concentration-response behavior
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The migrated cells were detected by a light microscope, and quantitative analysis of each image was performed using Image J software
However, the present review, which relies specifically on 24-h-averaged HR data, demonstrates that the degree of this positive chronotropic effect was underestimated when assessed by single time point pulse rate measurements
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