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The suspension was freeze-thawed 3x at 80C, sonicated 3x 15 s then spun at 12 k RPM for 30 min to pellet the insoluble material
The material is supplied in sealed laboratory vials to assist analytical investigations, assay development, and experimental protocols in professional environments where documentation and reproducibility are essential
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coli muropeptides [98] [Figure 2]
The treatment duration typically spans several weeks to months, depending on the skins response and the desired skin-lightening effects
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